NUCLEORA PLAYGROUND

Live biology tools.
Zero data leaves.

Four interactive instruments that run entirely in your browser — no server, no account, no data sent anywhere. Each one is a small window into what Nucleora computes in real time as you design.

01 / DRC SEQUENCE CHECK

Paste a sequence. Watch design rules run.

0 nt
GC —

Runs entirely in your browser — no data leaves this page.

02 / mRNA CONSTRUCT BUILDER

Assemble the construct. Watch the physics update.

Every element you pick propagates into length, structure, capping efficiency and manufacturability grade — before a nucleotide is ordered.

080100120150

Transcript length

—nt

5′ UTR ΔG (est.)

—

Manufacturability

—

Capping efficiency

—%

DRC PREVIEW · 3 RULES
DRC-01Kozak consensus
DRC-02Poly(A) length
DRC-035′ UTR structure

ΔG is a nearest-neighbour approximation — not a full MFE fold. Schematic uses √nt cartoon scale; junction coordinates are exact. Illustrative tool only.

03 / CODON OPTIMIZER

Protein in. Expression-ready DNA out.

Back-translates your protein to the codons your target host actually prefers — with homopolymer and GC-drift penalties applied.

30 residues · 31 codons
OPTIMIZED CDS · 5′ → 3′HOMO SAPIENS
Length
0nt
GC content
0.0%
CAI (est.)
0.000
Rare codons
0flagged
High usage ≥70% Medium 40–69% Rare <40% · flagged Hover a codon for detail

Runs in your browser — no sequence data leaves this page. CAI is the geometric mean of selected codon weights. Demonstration tool — not a substitute for full construct design.

04 / GC WINDOW ANALYSIS

Find the GC traps before the synthesizer does.

A sliding-window scan over your construct. Runs above 75% GC stall polymerase; runs below 30% melt during assembly. Both show up here before they appear in a failed synthesis report.

gc_window · sliding scan READY
201 nt DNA
30nt
10100

Narrow windows expose local spikes. Wide windows show regional drift.

65% CEILING 40% FLOOR 1007550250
40–65% optimal 30–40 / 65–75% marginal <30 / >75% critical hover to inspect
Overall GC—whole sequence
Problem windows—outside 40–65%
Longest clean run—contiguous in-spec
Windows scanned—step 1 nt
Sequence map · 60 nt / line · colored by local window

Runs entirely in your browser. Step size is 1 nt. Sequences >12,000 nt render only the first 12,000 in the map.

WANT THE FULL STACK?

These are demos. Nucleora runs everything locally, continuously, on your actual constructs.

Request early access →